太陽光模擬器的用於光毒性實驗
紫外線是與人體皮膚密切相關的一種重要的環境因素,根據波長不同，紫外線可分為長波紫外線(UVA，320-400nm)、中波紫外線(UVB，280-320nm)和短波紫外線(UVC，200-280nm)。能夠到達地球表麵的紫外線僅包括UVA和UVB，與UVA相比，同等劑量的UVB對皮膚的損傷強度是UVA的1000倍左右。紫外線照射皮膚細胞產生大量活性氧自由基，這些活性氧自由基與引起細胞凋亡密不可分。
實驗流程操作注意事項：
新的金屬鹵化物紫外線燈管應在使用前點亮約100小時，以獲得穩定的輻照強度。
根據Hoenle博士的說法，紫外線燈管的壽命時間（光譜穩定）至少為800小時。因此，建議記錄太陽光模擬器的使用時間。隻有在測試發射的紫外線輻照強度滿足的情況下，才允許延長使用。
1、將配備H1濾光片的SOL 500/SOL 3太陽光模擬器安裝在適當的穩定支架上，以便微調照射距離。
2、將SOL 500/SOL 3太陽光模擬器調節到輻照距離約60厘米左右。
3、打開太陽光模擬器開關，等待至少15分鍾，使用經過校準的UVA紫外輻射計測量通過細胞培養板蓋的紫外線輻照度。
4、調整SOL 500/SOL 3太陽光模擬器的距離，使UVA輻照度達到1.7 mW/cm²（每10分鍾輻照時間產生的劑量為1 J/cm²）
5、根據同時輻照的板數，檢查輻照區域輻照度分布是否均勻：可接受1.6-1.8 mW/cm2的範圍。
重要提示：如果在達到半個輻照時間（30分鍾）後（如chess Castleing），改變低輻照度和高輻照度板的位置，則可接受1.5和1.9 mW/cm2的大差值。
每次進行光毒性試驗前，應按照前麵步驟檢查SOL 500/SOL 3太陽光模擬器的輻照值。如果使用UVfushejijinxingdeceliangjieguodiyuguidingzhi，zeziwaixiandengguankenengyidadaoqishouming，huozhefushejiyouyugezhongyuanyinzaochengceliangpiancha。zaizhezhongqingkuangxia，yingshiyongdiertaixiangtongleixinghexiaozhunguodeziwaifushejijinxingjiaochajianzha，gaicankaofushejiweimeitianjinxingchuli，qieyingbaochizaiheianzhong。
德國Dr. Hoenle AG好樂集團成立於1976年，總公司為HONLE GROUP，是shi先xian進jin的de紫zi外wai工gong業ye的de供gong應ying商shang之zhi一yi。作zuo為wei紫zi外wai專zhuan家jia，開kai發fa，製zhi造zao和he分fen銷xiao各ge地di的de紫zi外wai線xian係xi統tong，紫zi外wai線xian燈deng和he紫zi外wai線xian測ce量liang設she備bei。這zhe套tao係xi統tong被bei用yong在zai交jiao叉cha反fan應ying活huo性xing物wu質zhi，表biao滅mieshajun以及太陽模擬。HONLE(HOENLE)產品在電子、微電子、精密工程和光學工業生產過程中使用，在印刷，汽車，航空和醫藥等領域的也有廣泛應用。
Calibration of the Solar Simulator
Note: New metal halide burners should be burned for ~100 hrs prior to first use to achieve a stable emittance.
According to Dr Hoenle the burner has a shelflife (in which the spectrum is stable) of at least 800 hrs. Recording of lamp usage hours is, therefore, recommended. Extended use is only acceptable if the emitted energy spectrum can be checked.
1. Mount the SOL 500 / SOL 3 lamp, equipped with a H1-filter, on any appropriate stable tripod allowing fine-adjustment of the exposure distance.
2. Adjust SOL 500 / SOL 3 to a distance of about 60 cm.
3. Switch the Lamp on, wait at least 15 minutes and measure irradiance through the lid of a cell culture plate using the calibrated UV radiometer (type 37, Dr. Hönle), equipped with an UVA-sensor of the same serial number
.
4. Adjust distance of SOL 500 / SOL 3 to achieve a UVA irradiance of 1.7 mW/cm² (The resulting dose will be 1 J/cm² per 10 min. exposure time)
5. According to the number of plates to be exposed concurrently, check the exposure area for equal distribution of irradiance:
A range of 1.6 - 1.8 mW/cm2 is acceptable.
Important: A maximum difference of 1.5 and 1.9 mW/cm2 can be accepted, if positions of the plates with low and high irradiance are changed after half time of the irradiation (30 minutes) is reached (like chess castling).
Calibration of the SOL 500 / SOL 3 shall be checked as described above each time before performing a phototoxicity assay. In case measurements with the UV radiometer reveal unexpected results, either the metal halide burner may have reached the end of it's shelflife, or the radiometer is de-calibrated due to various reasons. In this case, a second reference radiometer of the same type and calibration, which is has not been handled every day and kept in the dark shall be used for cross check.
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